Journal: BMC Genomics
Article Title: Characterization of Myf6 and association with growth traits in swimming crab ( Portunus trituberculatus )
doi: 10.1186/s12864-024-11181-6
Figure Lengend Snippet: Genomic structure and mRNA of Myf6 in swimming crab. The gene contains one exon and one intron. The 3′ and 5′ UTR (untranslated region, orange) and exon encoding the amino acid sequences (blue) are shown relative to their lengths in the cDNA sequences obtained
Article Snippet: After removing genomic DNA using DNase I (Fermentas, Vilnius, Lithuania), extracted total RNA of all samples was diluted to 200 ng/µL, and then used as a template for cDNA synthesis using M-MLV first-strand cDNA Synthesis Kit (Invitrogen). qRT-PCR was performed using the ChamQ Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China) to investigate the mRNA expression of Myf6 according to the manufacturer’s instructions.
Techniques: